Background and Objective : Cholangiocarcinoma (CCA) is a cancer of the biliary duct system that commonly invades surrounding liver tissue and extrahepatic bile ducts. It tends to have a poor prognosis at an early stage, high morbidity. Early diagnosis of malignant tumors is very important to prolong the survival of patients. The aim of this study was therefore to determine and analysis of bile spectral profiles obtained by FTIR spectroscopy may differentiate biomarker from patients with CCA and without bile duct malignant strictures.

Methods : This study was approved by the Human Ethics Committee (HE581251). Using FTIR spectroscopy study volunteers bile. The volunteers was performed in two groups of patients. The first group consisted of 30 patients with malignant biliary strictures. The second group consisted of 30 patients without malignant biliary strictures , but diagnostic with gallstone.

Results : The difference spectrum of lipid absorbance bands such as cholesterol, phospholipids, and creatine were higher in the malignant bile sample group than in the gall stone. The spectrum of bilirubin was no difference in the malignant bile sample and in the gall stone.

Conclusions : This work demonstrated the potential of using FTIR spectroscopy as a diagnostic tool for differentiate biomarker from patients bile with CCA and without bile duct malignant strictures.