Background and Objectives:Breast cancer is the second most common of death in female. ESA plays an important role in signaling pathway of breast cancer. The levels of ESA expression associate with prognosis, resistance of therapy and short survival time. At present, there is no data of the expression levels of cancer stem cells in breast cancer patients in Srinagarind hospital. Methods:The data of breast cancer patients (247 cases in total) in Srinagarind hospital collected during 2003-2016 were analysed. Formalin-fixed paraffin-embedded (FFPE) breast cancer tissues were investigated for the levels of ESA expression in 45 cases of triple negative subtype by immunohistochemistry. TIS score and H-score were performed to determine the levels of ESA expression.To evaluate the relationship of ESA expression levels and average survival time, the data were analysed by using univariate analysis and multivariate analysis. Results: Age, BMI, TNM staging had influence on average survival time. Patients with age more than or equal 41 years, obesity or TNM stage IV has high fatality risk. The levels of ESA expression in triple negative subtype had overexpression but it was not significantly different between two age groups (≥ 41 years vs 21-40 years). Conclusions:Age, BMI and TNM staging negatively related to average survival time in breast cancer patients and the effects were different in each subtype of breast cancer. Patients with triple negative subtype had overexpression of ESA.